HMM Applications in Light Sheet Microscopy

Supporting Both Scanning-Based and Static Light Sheet Systems with Precision Control and Real-Time Coordination

Light sheet microscopy generally falls into two major architectural categories:

1.   Scanning-based systems, which require complex synchronization; and

2.   Static illumination systems, which often generate significantly larger data volumes.

TLI’s HMM platform is designed to support both types through precise synchronization, flexible trigger control, and real-time data preprocessing — enabling researchers to streamline acquisition workflows and facilitate high-quality imaging.

 Scanning-Based Light Sheet Systems

These systems use two independent XY galvo modules: one to generate the light sheet and another to scan the sample or capture multi-view images. This architecture demands precise synchronization among galvos, cameras, and lasers to ensure accurate image alignment and data integrity.

 HMM enables:

• Multi-channel trigger outputs with programmable delays
• Synchronized control of scanning mirrors, light sources, and cameras
• Support for non-linear scan patterns and custom timing sequences
• Stable, repeatable acquisition for tissue imaging and multi-angle observations

 Static Illumination Light Sheet Systems

These systems create a stationary light sheet using optical components such as cylindrical lenses or beam-shaping optics. Although synchronization requirements are simpler, continuous or long-term imaging often generates large data volumes, necessitating efficient real-time control.

 HMM supports:

• Precise coordination of stage and camera timing
• In-line preprocessing, including background subtraction, artifact correction, or reslicing
• Real-time control of exposure timing and illumination triggering
• Streamlined acquisition workflows for high-throughput or live imaging applications

 Why HMM Is Suitable for Both Architectures

HMM is not an acquisition device itself; rather, it serves as a central control, coordination platform that ensures all system components operate in perfect synchrony. This allows users to maximize imaging efficiency, reduce errors, and simplify system integration.

 Comparison Summary

• In scanning-based systems, HMM manages complex multi-component timing to achieve accurate, high-fidelity acquisition.
• In static sheet systems, HMM improves workflow efficiency through real-time coordination and in-line preprocessing.

In both cases, HMM provides a stable, modular foundation that adapts to evolving system designs and experimental requirements.

Table 1 : HMM for Light Sheet Microscopy Control Card

Table 2 : Connector Block

Table 3 : Software

Table 4 : Others